Rotation project 1

Over the past 2 weeks our class has been undertaking training in the genetic engineering of yeast and E. coli cells with flourescent protein, which we have subsequently expressed and purified using chromatography, gel electrophoresis, and blotting techniques. We successfully cloned and purified green and red fluorescent proteins.

Next week we begin the first of our 12 week rotation projects. My project will involve the purification, kinetic assaying, and parameter fitting of a subgroup of enzymes that are involved in a so-far uncharted part of the yeast metabolic pathway. We will present a poster of our project to our colleagues in the systems biology program at Warwick University.